Chromatin Immunoprecipitation:Article Title: Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-POU5F1 C-10 (1:400; Santa Cruz, #sc-5279) Anti-NANOG P1-2D8 (1:200, Millipore, #MABD24) Anti-PAX6 (1:200; Abcam #ab5790) Anti-Nestin (1:200; R&D Systems, #MAB1259) Anti-MAP2 (1:1000; Abcam, #ab92434) Anti-CHAT (1:200; Abcam, #ab6168) Anti-cTNT (1:5; CT3, deposited to the DSHB by Lin, J.J-C.) Anti-ACTN2 (1:800; Sigma-Aldrich #A7811) Anti-ZNF598 (1:1000, #ab135921 Abcam) Anti-PELO F-4 (1:1000, #sc-393418 Santa Cruz Biotechnology) Anti-HBS1L (1:1000, #HPA029729 Atlas Antibodies) Anti-ASCC3 (1:1000, #A304-015A Bethyl Laboratories) Anti-eIF2α (1:1000, #9722 Cell Signaling) Anti-eIF2α-p S51 (1:1000, #ab32157 Abcam) Anti-p38 (1:1000, #9212 Cell Signaling) Anti-p38-p Thr180/Tyr182 (1:1000, #9211 Cell Signaling) Anti-uS10 (1:1000, #ab151550 Abcam) Anti-eS10 (1:1000, #ab133776, Abcam) Anti-uS5 (1:1000, #A303-794A Bethyl Laboratories) Anti-uS3 (1:1000, #A303-840A Bethyl Laboratories) Goat anti-mouse Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11001) Goat anti-rabbit Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11034) Goat anti-mouse Alexa Fluor 633 (1:500; Thermo Fisher Scientific, #A-21052) Anti-mouse IgG-HRP, 1:4000; Dianova, #115-035-003 Anti-rabbit IgG-HRP (1:4000; Dianova, #111-035-003) Validation Anti-POU5F1 C-10 (Santa Cruz, #sc-5279): negative staining of cells that are not expressing the marker in Gao, Behrens et al., Nature Cell Biology 2024 . .. Anti-P1-2D8 (1:200, Millipore, #MABD24): according to the manufacturer, this monoclonal antibody is suitable for detecting human NANOG by immunohistochemistry; cited in 40 publications (https://www.sigmaaldrich.com/GB/en/product/mm/mabd24).
Article Title: Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-POU5F1 C-10 (1:400; Santa Cruz, #sc-5279) Anti-NANOG P1-2D8 (1:200, Millipore, #MABD24) Anti-PAX6 (1:200; Abcam #ab5790) Anti-Nestin (1:200; R&D Systems, #MAB1259) Anti-MAP2 (1:1000; Abcam, #ab92434) Anti-CHAT (1:200; Abcam, #ab6168) Anti-cTNT (1:5; CT3, deposited to the DSHB by Lin, J.J-C.) Anti-ACTN2 (1:800; Sigma-Aldrich #A7811) Anti-ZNF598 (1:1000, #ab135921 Abcam) Anti-PELO F-4 (1:1000, #sc-393418 Santa Cruz Biotechnology) Anti-HBS1L (1:1000, #HPA029729 Atlas Antibodies) Anti-ASCC3 (1:1000, #A304-015A Bethyl Laboratories) Anti-eIF2α (1:1000, #9722 Cell Signaling) Anti-eIF2α-p S51 (1:1000, #ab32157 Abcam) Anti-p38 (1:1000, #9212 Cell Signaling) Anti-p38-p Thr180/Tyr182 (1:1000, #9211 Cell Signaling) Anti-uS10 (1:1000, #ab151550 Abcam) Anti-eS10 (1:1000, #ab133776, Abcam) Anti-uS5 (1:1000, #A303-794A Bethyl Laboratories) Anti-uS3 (1:1000, #A303-840A Bethyl Laboratories) Goat anti-mouse Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11001) Goat anti-rabbit Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11034) Goat anti-mouse Alexa Fluor 633 (1:500; Thermo Fisher Scientific, #A-21052) Anti-mouse IgG-HRP, 1:4000; Dianova, #115-035-003 Anti-rabbit IgG-HRP (1:4000; Dianova, #111-035-003) Validation Anti-POU5F1 C-10 (Santa Cruz, #sc-5279): negative staining of cells that are not expressing the marker in Gao, Behrens et al., Nature Cell Biology 2024 . .. Anti-P1-2D8 (1:200, Millipore, #MABD24): according to the manufacturer, this monoclonal antibody is suitable for detecting human NANOG by immunohistochemistry; cited in 40 publications (https://www.sigmaaldrich.com/GB/en/product/mm/mabd24).
Flow Cytometry:Article Title: Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-POU5F1 C-10 (1:400; Santa Cruz, #sc-5279) Anti-NANOG P1-2D8 (1:200, Millipore, #MABD24) Anti-PAX6 (1:200; Abcam #ab5790) Anti-Nestin (1:200; R&D Systems, #MAB1259) Anti-MAP2 (1:1000; Abcam, #ab92434) Anti-CHAT (1:200; Abcam, #ab6168) Anti-cTNT (1:5; CT3, deposited to the DSHB by Lin, J.J-C.) Anti-ACTN2 (1:800; Sigma-Aldrich #A7811) Anti-ZNF598 (1:1000, #ab135921 Abcam) Anti-PELO F-4 (1:1000, #sc-393418 Santa Cruz Biotechnology) Anti-HBS1L (1:1000, #HPA029729 Atlas Antibodies) Anti-ASCC3 (1:1000, #A304-015A Bethyl Laboratories) Anti-eIF2α (1:1000, #9722 Cell Signaling) Anti-eIF2α-p S51 (1:1000, #ab32157 Abcam) Anti-p38 (1:1000, #9212 Cell Signaling) Anti-p38-p Thr180/Tyr182 (1:1000, #9211 Cell Signaling) Anti-uS10 (1:1000, #ab151550 Abcam) Anti-eS10 (1:1000, #ab133776, Abcam) Anti-uS5 (1:1000, #A303-794A Bethyl Laboratories) Anti-uS3 (1:1000, #A303-840A Bethyl Laboratories) Goat anti-mouse Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11001) Goat anti-rabbit Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11034) Goat anti-mouse Alexa Fluor 633 (1:500; Thermo Fisher Scientific, #A-21052) Anti-mouse IgG-HRP, 1:4000; Dianova, #115-035-003 Anti-rabbit IgG-HRP (1:4000; Dianova, #111-035-003) Validation Anti-POU5F1 C-10 (Santa Cruz, #sc-5279): negative staining of cells that are not expressing the marker in Gao, Behrens et al., Nature Cell Biology 2024 . .. Anti-P1-2D8 (1:200, Millipore, #MABD24): according to the manufacturer, this monoclonal antibody is suitable for detecting human NANOG by immunohistochemistry; cited in 40 publications (https://www.sigmaaldrich.com/GB/en/product/mm/mabd24).
Article Title: Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-POU5F1 C-10 (1:400; Santa Cruz, #sc-5279) Anti-NANOG P1-2D8 (1:200, Millipore, #MABD24) Anti-PAX6 (1:200; Abcam #ab5790) Anti-Nestin (1:200; R&D Systems, #MAB1259) Anti-MAP2 (1:1000; Abcam, #ab92434) Anti-CHAT (1:200; Abcam, #ab6168) Anti-cTNT (1:5; CT3, deposited to the DSHB by Lin, J.J-C.) Anti-ACTN2 (1:800; Sigma-Aldrich #A7811) Anti-ZNF598 (1:1000, #ab135921 Abcam) Anti-PELO F-4 (1:1000, #sc-393418 Santa Cruz Biotechnology) Anti-HBS1L (1:1000, #HPA029729 Atlas Antibodies) Anti-ASCC3 (1:1000, #A304-015A Bethyl Laboratories) Anti-eIF2α (1:1000, #9722 Cell Signaling) Anti-eIF2α-p S51 (1:1000, #ab32157 Abcam) Anti-p38 (1:1000, #9212 Cell Signaling) Anti-p38-p Thr180/Tyr182 (1:1000, #9211 Cell Signaling) Anti-uS10 (1:1000, #ab151550 Abcam) Anti-eS10 (1:1000, #ab133776, Abcam) Anti-uS5 (1:1000, #A303-794A Bethyl Laboratories) Anti-uS3 (1:1000, #A303-840A Bethyl Laboratories) Goat anti-mouse Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11001) Goat anti-rabbit Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11034) Goat anti-mouse Alexa Fluor 633 (1:500; Thermo Fisher Scientific, #A-21052) Anti-mouse IgG-HRP, 1:4000; Dianova, #115-035-003 Anti-rabbit IgG-HRP (1:4000; Dianova, #111-035-003) Validation Anti-POU5F1 C-10 (Santa Cruz, #sc-5279): negative staining of cells that are not expressing the marker in Gao, Behrens et al., Nature Cell Biology 2024 . .. Anti-P1-2D8 (1:200, Millipore, #MABD24): according to the manufacturer, this monoclonal antibody is suitable for detecting human NANOG by immunohistochemistry; cited in 40 publications (https://www.sigmaaldrich.com/GB/en/product/mm/mabd24).
Magnetic Resonance Imaging:Article Title: Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-POU5F1 C-10 (1:400; Santa Cruz, #sc-5279) Anti-NANOG P1-2D8 (1:200, Millipore, #MABD24) Anti-PAX6 (1:200; Abcam #ab5790) Anti-Nestin (1:200; R&D Systems, #MAB1259) Anti-MAP2 (1:1000; Abcam, #ab92434) Anti-CHAT (1:200; Abcam, #ab6168) Anti-cTNT (1:5; CT3, deposited to the DSHB by Lin, J.J-C.) Anti-ACTN2 (1:800; Sigma-Aldrich #A7811) Anti-ZNF598 (1:1000, #ab135921 Abcam) Anti-PELO F-4 (1:1000, #sc-393418 Santa Cruz Biotechnology) Anti-HBS1L (1:1000, #HPA029729 Atlas Antibodies) Anti-ASCC3 (1:1000, #A304-015A Bethyl Laboratories) Anti-eIF2α (1:1000, #9722 Cell Signaling) Anti-eIF2α-p S51 (1:1000, #ab32157 Abcam) Anti-p38 (1:1000, #9212 Cell Signaling) Anti-p38-p Thr180/Tyr182 (1:1000, #9211 Cell Signaling) Anti-uS10 (1:1000, #ab151550 Abcam) Anti-eS10 (1:1000, #ab133776, Abcam) Anti-uS5 (1:1000, #A303-794A Bethyl Laboratories) Anti-uS3 (1:1000, #A303-840A Bethyl Laboratories) Goat anti-mouse Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11001) Goat anti-rabbit Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11034) Goat anti-mouse Alexa Fluor 633 (1:500; Thermo Fisher Scientific, #A-21052) Anti-mouse IgG-HRP, 1:4000; Dianova, #115-035-003 Anti-rabbit IgG-HRP (1:4000; Dianova, #111-035-003) Validation Anti-POU5F1 C-10 (Santa Cruz, #sc-5279): negative staining of cells that are not expressing the marker in Gao, Behrens et al., Nature Cell Biology 2024 . .. Anti-P1-2D8 (1:200, Millipore, #MABD24): according to the manufacturer, this monoclonal antibody is suitable for detecting human NANOG by immunohistochemistry; cited in 40 publications (https://www.sigmaaldrich.com/GB/en/product/mm/mabd24).
Article Title: Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-POU5F1 C-10 (1:400; Santa Cruz, #sc-5279) Anti-NANOG P1-2D8 (1:200, Millipore, #MABD24) Anti-PAX6 (1:200; Abcam #ab5790) Anti-Nestin (1:200; R&D Systems, #MAB1259) Anti-MAP2 (1:1000; Abcam, #ab92434) Anti-CHAT (1:200; Abcam, #ab6168) Anti-cTNT (1:5; CT3, deposited to the DSHB by Lin, J.J-C.) Anti-ACTN2 (1:800; Sigma-Aldrich #A7811) Anti-ZNF598 (1:1000, #ab135921 Abcam) Anti-PELO F-4 (1:1000, #sc-393418 Santa Cruz Biotechnology) Anti-HBS1L (1:1000, #HPA029729 Atlas Antibodies) Anti-ASCC3 (1:1000, #A304-015A Bethyl Laboratories) Anti-eIF2α (1:1000, #9722 Cell Signaling) Anti-eIF2α-p S51 (1:1000, #ab32157 Abcam) Anti-p38 (1:1000, #9212 Cell Signaling) Anti-p38-p Thr180/Tyr182 (1:1000, #9211 Cell Signaling) Anti-uS10 (1:1000, #ab151550 Abcam) Anti-eS10 (1:1000, #ab133776, Abcam) Anti-uS5 (1:1000, #A303-794A Bethyl Laboratories) Anti-uS3 (1:1000, #A303-840A Bethyl Laboratories) Goat anti-mouse Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11001) Goat anti-rabbit Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11034) Goat anti-mouse Alexa Fluor 633 (1:500; Thermo Fisher Scientific, #A-21052) Anti-mouse IgG-HRP, 1:4000; Dianova, #115-035-003 Anti-rabbit IgG-HRP (1:4000; Dianova, #111-035-003) Validation Anti-POU5F1 C-10 (Santa Cruz, #sc-5279): negative staining of cells that are not expressing the marker in Gao, Behrens et al., Nature Cell Biology 2024 . .. Anti-P1-2D8 (1:200, Millipore, #MABD24): according to the manufacturer, this monoclonal antibody is suitable for detecting human NANOG by immunohistochemistry; cited in 40 publications (https://www.sigmaaldrich.com/GB/en/product/mm/mabd24).
Biomarker Discovery:Article Title: Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-POU5F1 C-10 (1:400; Santa Cruz, #sc-5279) Anti-NANOG P1-2D8 (1:200, Millipore, #MABD24) Anti-PAX6 (1:200; Abcam #ab5790) Anti-Nestin (1:200; R&D Systems, #MAB1259) Anti-MAP2 (1:1000; Abcam, #ab92434) Anti-CHAT (1:200; Abcam, #ab6168) Anti-cTNT (1:5; CT3, deposited to the DSHB by Lin, J.J-C.) Anti-ACTN2 (1:800; Sigma-Aldrich #A7811) Anti-ZNF598 (1:1000, #ab135921 Abcam) Anti-PELO F-4 (1:1000, #sc-393418 Santa Cruz Biotechnology) Anti-HBS1L (1:1000, #HPA029729 Atlas Antibodies) Anti-ASCC3 (1:1000, #A304-015A Bethyl Laboratories) Anti-eIF2α (1:1000, #9722 Cell Signaling) Anti-eIF2α-p S51 (1:1000, #ab32157 Abcam) Anti-p38 (1:1000, #9212 Cell Signaling) Anti-p38-p Thr180/Tyr182 (1:1000, #9211 Cell Signaling) Anti-uS10 (1:1000, #ab151550 Abcam) Anti-eS10 (1:1000, #ab133776, Abcam) Anti-uS5 (1:1000, #A303-794A Bethyl Laboratories) Anti-uS3 (1:1000, #A303-840A Bethyl Laboratories) Goat anti-mouse Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11001) Goat anti-rabbit Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11034) Goat anti-mouse Alexa Fluor 633 (1:500; Thermo Fisher Scientific, #A-21052) Anti-mouse IgG-HRP, 1:4000; Dianova, #115-035-003 Anti-rabbit IgG-HRP (1:4000; Dianova, #111-035-003) Validation Anti-POU5F1 C-10 (Santa Cruz, #sc-5279): negative staining of cells that are not expressing the marker in Gao, Behrens et al., Nature Cell Biology 2024 . .. Anti-P1-2D8 (1:200, Millipore, #MABD24): according to the manufacturer, this monoclonal antibody is suitable for detecting human NANOG by immunohistochemistry; cited in 40 publications (https://www.sigmaaldrich.com/GB/en/product/mm/mabd24).
Article Title: Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-POU5F1 C-10 (1:400; Santa Cruz, #sc-5279) Anti-NANOG P1-2D8 (1:200, Millipore, #MABD24) Anti-PAX6 (1:200; Abcam #ab5790) Anti-Nestin (1:200; R&D Systems, #MAB1259) Anti-MAP2 (1:1000; Abcam, #ab92434) Anti-CHAT (1:200; Abcam, #ab6168) Anti-cTNT (1:5; CT3, deposited to the DSHB by Lin, J.J-C.) Anti-ACTN2 (1:800; Sigma-Aldrich #A7811) Anti-ZNF598 (1:1000, #ab135921 Abcam) Anti-PELO F-4 (1:1000, #sc-393418 Santa Cruz Biotechnology) Anti-HBS1L (1:1000, #HPA029729 Atlas Antibodies) Anti-ASCC3 (1:1000, #A304-015A Bethyl Laboratories) Anti-eIF2α (1:1000, #9722 Cell Signaling) Anti-eIF2α-p S51 (1:1000, #ab32157 Abcam) Anti-p38 (1:1000, #9212 Cell Signaling) Anti-p38-p Thr180/Tyr182 (1:1000, #9211 Cell Signaling) Anti-uS10 (1:1000, #ab151550 Abcam) Anti-eS10 (1:1000, #ab133776, Abcam) Anti-uS5 (1:1000, #A303-794A Bethyl Laboratories) Anti-uS3 (1:1000, #A303-840A Bethyl Laboratories) Goat anti-mouse Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11001) Goat anti-rabbit Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11034) Goat anti-mouse Alexa Fluor 633 (1:500; Thermo Fisher Scientific, #A-21052) Anti-mouse IgG-HRP, 1:4000; Dianova, #115-035-003 Anti-rabbit IgG-HRP (1:4000; Dianova, #111-035-003) Validation Anti-POU5F1 C-10 (Santa Cruz, #sc-5279): negative staining of cells that are not expressing the marker in Gao, Behrens et al., Nature Cell Biology 2024 . .. Anti-P1-2D8 (1:200, Millipore, #MABD24): according to the manufacturer, this monoclonal antibody is suitable for detecting human NANOG by immunohistochemistry; cited in 40 publications (https://www.sigmaaldrich.com/GB/en/product/mm/mabd24).
Negative Staining:Article Title: Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-POU5F1 C-10 (1:400; Santa Cruz, #sc-5279) Anti-NANOG P1-2D8 (1:200, Millipore, #MABD24) Anti-PAX6 (1:200; Abcam #ab5790) Anti-Nestin (1:200; R&D Systems, #MAB1259) Anti-MAP2 (1:1000; Abcam, #ab92434) Anti-CHAT (1:200; Abcam, #ab6168) Anti-cTNT (1:5; CT3, deposited to the DSHB by Lin, J.J-C.) Anti-ACTN2 (1:800; Sigma-Aldrich #A7811) Anti-ZNF598 (1:1000, #ab135921 Abcam) Anti-PELO F-4 (1:1000, #sc-393418 Santa Cruz Biotechnology) Anti-HBS1L (1:1000, #HPA029729 Atlas Antibodies) Anti-ASCC3 (1:1000, #A304-015A Bethyl Laboratories) Anti-eIF2α (1:1000, #9722 Cell Signaling) Anti-eIF2α-p S51 (1:1000, #ab32157 Abcam) Anti-p38 (1:1000, #9212 Cell Signaling) Anti-p38-p Thr180/Tyr182 (1:1000, #9211 Cell Signaling) Anti-uS10 (1:1000, #ab151550 Abcam) Anti-eS10 (1:1000, #ab133776, Abcam) Anti-uS5 (1:1000, #A303-794A Bethyl Laboratories) Anti-uS3 (1:1000, #A303-840A Bethyl Laboratories) Goat anti-mouse Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11001) Goat anti-rabbit Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11034) Goat anti-mouse Alexa Fluor 633 (1:500; Thermo Fisher Scientific, #A-21052) Anti-mouse IgG-HRP, 1:4000; Dianova, #115-035-003 Anti-rabbit IgG-HRP (1:4000; Dianova, #111-035-003) Validation Anti-POU5F1 C-10 (Santa Cruz, #sc-5279): negative staining of cells that are not expressing the marker in Gao, Behrens et al., Nature Cell Biology 2024 . .. Anti-P1-2D8 (1:200, Millipore, #MABD24): according to the manufacturer, this monoclonal antibody is suitable for detecting human NANOG by immunohistochemistry; cited in 40 publications (https://www.sigmaaldrich.com/GB/en/product/mm/mabd24).
Article Title: Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-POU5F1 C-10 (1:400; Santa Cruz, #sc-5279) Anti-NANOG P1-2D8 (1:200, Millipore, #MABD24) Anti-PAX6 (1:200; Abcam #ab5790) Anti-Nestin (1:200; R&D Systems, #MAB1259) Anti-MAP2 (1:1000; Abcam, #ab92434) Anti-CHAT (1:200; Abcam, #ab6168) Anti-cTNT (1:5; CT3, deposited to the DSHB by Lin, J.J-C.) Anti-ACTN2 (1:800; Sigma-Aldrich #A7811) Anti-ZNF598 (1:1000, #ab135921 Abcam) Anti-PELO F-4 (1:1000, #sc-393418 Santa Cruz Biotechnology) Anti-HBS1L (1:1000, #HPA029729 Atlas Antibodies) Anti-ASCC3 (1:1000, #A304-015A Bethyl Laboratories) Anti-eIF2α (1:1000, #9722 Cell Signaling) Anti-eIF2α-p S51 (1:1000, #ab32157 Abcam) Anti-p38 (1:1000, #9212 Cell Signaling) Anti-p38-p Thr180/Tyr182 (1:1000, #9211 Cell Signaling) Anti-uS10 (1:1000, #ab151550 Abcam) Anti-eS10 (1:1000, #ab133776, Abcam) Anti-uS5 (1:1000, #A303-794A Bethyl Laboratories) Anti-uS3 (1:1000, #A303-840A Bethyl Laboratories) Goat anti-mouse Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11001) Goat anti-rabbit Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11034) Goat anti-mouse Alexa Fluor 633 (1:500; Thermo Fisher Scientific, #A-21052) Anti-mouse IgG-HRP, 1:4000; Dianova, #115-035-003 Anti-rabbit IgG-HRP (1:4000; Dianova, #111-035-003) Validation Anti-POU5F1 C-10 (Santa Cruz, #sc-5279): negative staining of cells that are not expressing the marker in Gao, Behrens et al., Nature Cell Biology 2024 . .. Anti-P1-2D8 (1:200, Millipore, #MABD24): according to the manufacturer, this monoclonal antibody is suitable for detecting human NANOG by immunohistochemistry; cited in 40 publications (https://www.sigmaaldrich.com/GB/en/product/mm/mabd24).
Expressing:Article Title: Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-POU5F1 C-10 (1:400; Santa Cruz, #sc-5279) Anti-NANOG P1-2D8 (1:200, Millipore, #MABD24) Anti-PAX6 (1:200; Abcam #ab5790) Anti-Nestin (1:200; R&D Systems, #MAB1259) Anti-MAP2 (1:1000; Abcam, #ab92434) Anti-CHAT (1:200; Abcam, #ab6168) Anti-cTNT (1:5; CT3, deposited to the DSHB by Lin, J.J-C.) Anti-ACTN2 (1:800; Sigma-Aldrich #A7811) Anti-ZNF598 (1:1000, #ab135921 Abcam) Anti-PELO F-4 (1:1000, #sc-393418 Santa Cruz Biotechnology) Anti-HBS1L (1:1000, #HPA029729 Atlas Antibodies) Anti-ASCC3 (1:1000, #A304-015A Bethyl Laboratories) Anti-eIF2α (1:1000, #9722 Cell Signaling) Anti-eIF2α-p S51 (1:1000, #ab32157 Abcam) Anti-p38 (1:1000, #9212 Cell Signaling) Anti-p38-p Thr180/Tyr182 (1:1000, #9211 Cell Signaling) Anti-uS10 (1:1000, #ab151550 Abcam) Anti-eS10 (1:1000, #ab133776, Abcam) Anti-uS5 (1:1000, #A303-794A Bethyl Laboratories) Anti-uS3 (1:1000, #A303-840A Bethyl Laboratories) Goat anti-mouse Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11001) Goat anti-rabbit Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11034) Goat anti-mouse Alexa Fluor 633 (1:500; Thermo Fisher Scientific, #A-21052) Anti-mouse IgG-HRP, 1:4000; Dianova, #115-035-003 Anti-rabbit IgG-HRP (1:4000; Dianova, #111-035-003) Validation Anti-POU5F1 C-10 (Santa Cruz, #sc-5279): negative staining of cells that are not expressing the marker in Gao, Behrens et al., Nature Cell Biology 2024 . .. Anti-P1-2D8 (1:200, Millipore, #MABD24): according to the manufacturer, this monoclonal antibody is suitable for detecting human NANOG by immunohistochemistry; cited in 40 publications (https://www.sigmaaldrich.com/GB/en/product/mm/mabd24).
Article Title: Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-POU5F1 C-10 (1:400; Santa Cruz, #sc-5279) Anti-NANOG P1-2D8 (1:200, Millipore, #MABD24) Anti-PAX6 (1:200; Abcam #ab5790) Anti-Nestin (1:200; R&D Systems, #MAB1259) Anti-MAP2 (1:1000; Abcam, #ab92434) Anti-CHAT (1:200; Abcam, #ab6168) Anti-cTNT (1:5; CT3, deposited to the DSHB by Lin, J.J-C.) Anti-ACTN2 (1:800; Sigma-Aldrich #A7811) Anti-ZNF598 (1:1000, #ab135921 Abcam) Anti-PELO F-4 (1:1000, #sc-393418 Santa Cruz Biotechnology) Anti-HBS1L (1:1000, #HPA029729 Atlas Antibodies) Anti-ASCC3 (1:1000, #A304-015A Bethyl Laboratories) Anti-eIF2α (1:1000, #9722 Cell Signaling) Anti-eIF2α-p S51 (1:1000, #ab32157 Abcam) Anti-p38 (1:1000, #9212 Cell Signaling) Anti-p38-p Thr180/Tyr182 (1:1000, #9211 Cell Signaling) Anti-uS10 (1:1000, #ab151550 Abcam) Anti-eS10 (1:1000, #ab133776, Abcam) Anti-uS5 (1:1000, #A303-794A Bethyl Laboratories) Anti-uS3 (1:1000, #A303-840A Bethyl Laboratories) Goat anti-mouse Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11001) Goat anti-rabbit Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11034) Goat anti-mouse Alexa Fluor 633 (1:500; Thermo Fisher Scientific, #A-21052) Anti-mouse IgG-HRP, 1:4000; Dianova, #115-035-003 Anti-rabbit IgG-HRP (1:4000; Dianova, #111-035-003) Validation Anti-POU5F1 C-10 (Santa Cruz, #sc-5279): negative staining of cells that are not expressing the marker in Gao, Behrens et al., Nature Cell Biology 2024 . .. Anti-P1-2D8 (1:200, Millipore, #MABD24): according to the manufacturer, this monoclonal antibody is suitable for detecting human NANOG by immunohistochemistry; cited in 40 publications (https://www.sigmaaldrich.com/GB/en/product/mm/mabd24).
Marker:Article Title: Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-POU5F1 C-10 (1:400; Santa Cruz, #sc-5279) Anti-NANOG P1-2D8 (1:200, Millipore, #MABD24) Anti-PAX6 (1:200; Abcam #ab5790) Anti-Nestin (1:200; R&D Systems, #MAB1259) Anti-MAP2 (1:1000; Abcam, #ab92434) Anti-CHAT (1:200; Abcam, #ab6168) Anti-cTNT (1:5; CT3, deposited to the DSHB by Lin, J.J-C.) Anti-ACTN2 (1:800; Sigma-Aldrich #A7811) Anti-ZNF598 (1:1000, #ab135921 Abcam) Anti-PELO F-4 (1:1000, #sc-393418 Santa Cruz Biotechnology) Anti-HBS1L (1:1000, #HPA029729 Atlas Antibodies) Anti-ASCC3 (1:1000, #A304-015A Bethyl Laboratories) Anti-eIF2α (1:1000, #9722 Cell Signaling) Anti-eIF2α-p S51 (1:1000, #ab32157 Abcam) Anti-p38 (1:1000, #9212 Cell Signaling) Anti-p38-p Thr180/Tyr182 (1:1000, #9211 Cell Signaling) Anti-uS10 (1:1000, #ab151550 Abcam) Anti-eS10 (1:1000, #ab133776, Abcam) Anti-uS5 (1:1000, #A303-794A Bethyl Laboratories) Anti-uS3 (1:1000, #A303-840A Bethyl Laboratories) Goat anti-mouse Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11001) Goat anti-rabbit Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11034) Goat anti-mouse Alexa Fluor 633 (1:500; Thermo Fisher Scientific, #A-21052) Anti-mouse IgG-HRP, 1:4000; Dianova, #115-035-003 Anti-rabbit IgG-HRP (1:4000; Dianova, #111-035-003) Validation Anti-POU5F1 C-10 (Santa Cruz, #sc-5279): negative staining of cells that are not expressing the marker in Gao, Behrens et al., Nature Cell Biology 2024 . .. Anti-P1-2D8 (1:200, Millipore, #MABD24): according to the manufacturer, this monoclonal antibody is suitable for detecting human NANOG by immunohistochemistry; cited in 40 publications (https://www.sigmaaldrich.com/GB/en/product/mm/mabd24).
Article Title: Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-POU5F1 C-10 (1:400; Santa Cruz, #sc-5279) Anti-NANOG P1-2D8 (1:200, Millipore, #MABD24) Anti-PAX6 (1:200; Abcam #ab5790) Anti-Nestin (1:200; R&D Systems, #MAB1259) Anti-MAP2 (1:1000; Abcam, #ab92434) Anti-CHAT (1:200; Abcam, #ab6168) Anti-cTNT (1:5; CT3, deposited to the DSHB by Lin, J.J-C.) Anti-ACTN2 (1:800; Sigma-Aldrich #A7811) Anti-ZNF598 (1:1000, #ab135921 Abcam) Anti-PELO F-4 (1:1000, #sc-393418 Santa Cruz Biotechnology) Anti-HBS1L (1:1000, #HPA029729 Atlas Antibodies) Anti-ASCC3 (1:1000, #A304-015A Bethyl Laboratories) Anti-eIF2α (1:1000, #9722 Cell Signaling) Anti-eIF2α-p S51 (1:1000, #ab32157 Abcam) Anti-p38 (1:1000, #9212 Cell Signaling) Anti-p38-p Thr180/Tyr182 (1:1000, #9211 Cell Signaling) Anti-uS10 (1:1000, #ab151550 Abcam) Anti-eS10 (1:1000, #ab133776, Abcam) Anti-uS5 (1:1000, #A303-794A Bethyl Laboratories) Anti-uS3 (1:1000, #A303-840A Bethyl Laboratories) Goat anti-mouse Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11001) Goat anti-rabbit Alexa Fluor 488 (1:2000; Thermo Fisher Scientific, #A-11034) Goat anti-mouse Alexa Fluor 633 (1:500; Thermo Fisher Scientific, #A-21052) Anti-mouse IgG-HRP, 1:4000; Dianova, #115-035-003 Anti-rabbit IgG-HRP (1:4000; Dianova, #111-035-003) Validation Anti-POU5F1 C-10 (Santa Cruz, #sc-5279): negative staining of cells that are not expressing the marker in Gao, Behrens et al., Nature Cell Biology 2024 . .. Anti-P1-2D8 (1:200, Millipore, #MABD24): according to the manufacturer, this monoclonal antibody is suitable for detecting human NANOG by immunohistochemistry; cited in 40 publications (https://www.sigmaaldrich.com/GB/en/product/mm/mabd24).
other:Article Title: Selective gene expression maintains human tRNA anticodon pools during differentiation.
Article Snippet: Anti-PAX6 (Abcam #ab5790): according to the manufactirer, this polyclonal antibody is suitable for detecting PAX6 from mouse, human, rat, and monkey by immunofluorescence; cited in >100 publications (https://www.abcam.com/products/primary-antibodies/ pax6-antibody-ab5790.html?productWallTab=ShowAll) AAnti-Nestin (R&D Systems, #MAB1259): according to the manufactirer, this monoclonal antibody detects human nestin by immunofluorescence; cited in >100 publications (https://www.rndsystems.com/products/human-nestinantibody-196908_mab1259?gclid=Cj0KCQjw4NujBhC5ARIsAF4Iv6eJnyTOhCazimeegmTVBXGL9pW6doy4o12apC7F5i93ffSmtPhOxUaAtgNEALw_wcB&gclsrc=aw.ds#product-details) Anti-MAP2 (Abcam, #ab92434): according to the manufactirer, this polyclonal antibody detects human MAP2 by immunofluorescence; cited in >45 publications (https://www.abcam.com/products/primary-antibodies/map2-antibodyab92434.html) Anti-CHAT (Abcam, #ab6168): according to the manufactirer, this polyclonal antibody was raised against a peptide corresponding to amino acids 168-189 of Choline Acetyltransferase and reacts with the protein in immunohistochemitry; cited in >13 publications (https://www.abcam.com/products/primary-antibodies/choline-acetyltransferase-antibody-ab6168.html) Anti-cTNT (CT3, deposited to the DSHB by Lin, J.J-C.): initially published in initially published in: Jin, J., Lin, J.-C., and Lin, J.J.-C. (1990).
Article Title: Selective gene expression maintains human tRNA anticodon pools during differentiation
Article Snippet: Anti-NANOG P1-2D8 (DSHB Hybridoma Product PCRP-NANOGP1-2D8): according to the manufacturer, this monoclonal antibody was raised against amino acids 1-127 of the human NANOG protein and is recommended for detecting human NANOG by immunofluorescence (https://dshb.biology.uiowa.edu/PCRP-NANOGP1-2D8).
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